International Journal of Pharmaceutical Investigation logo
Submit
International Journal of Pharmaceutical InvestigationACADEMIC PUBLISHING
Article

Detection of Extended Spectrum Beta Lactamases (ESBLs) Producing Enterobacteriaceae Family from Urinary Tract Infection (UTI) Patients

1★, 2, 3★ Corresponding author

  1. 1Department of Microbiology, Pachamuthu College of Arts and Science for Womens, Gundalapatti, Dharmapuri, Tamil Nadu, INDIA.
  2. 2Department of Microbiology, Government Arts College for Men, Krishnagiri, Tamil Nadu, INDIA.
  3. 3Department of Microbiology, Muthayammal College of Arts and Science College, Rasipuram, Tamil Nadu, INDIA.

CORRESPONDENCE

Devipalanisamy D

Department of Microbiology, Pachamuthu College of Arts and Science for Womens, Gundalapatti, Dharmapuri, Tamil Nadu, INDIA.

devibalapdf@gmail.com

Received: 28-11-2020; Revised: 15-12-2020; Accepted: 01-01-2021.

Volume 11, Issue 1 · pp. 113-117 · 2021 · DOI: 10.5530/ijpi.2021.1.21

ABSTRACT

Background: Extended spectrum beta lactamase (ESBLs) are betalactamase capable of conferring bacterial resistance to the first, second and third-generation Cephalosporin’s but these are inhibited by lactamase inhibitors such as clavulanic acid. The ESBLs are typically plasmidmediated enzymes, mostly produced by E. coli and Klebsiella pneumonia Enterobacteriaceae family. ESBLs enzymes were discovered in Germany in 1983 from Klebsiella pneumonia. ESBL-producing gram negative bacteria have been responsible for numerous outbreaks of infection throughout the several counties and region challenging infection control issues. Different phenotypic test for ESBL detection have been developed, which are easy to use and cost effective.Materials and Methods: In this study, 10 Urine samples were collected from Government Hospital, Dharmapuri, Tamil Nadu. Collected clinical samples were isolated and identified by standard microbiological method. The antibiotic susceptibility testing was performed by the disc-diffusion method. The preliminary phenotypic detection of ESBLs was carried out by combination disc-diffusion test (CDDT) Performed using Kirby- bauer disc diffusion method on muller hinton agar.Results: Totally 10 clinical samples were collected and investigated Among 7 clinical samples of positive of Urinary tract infection (UTI), 20 micro-organisms belonging to 3 genus were isolated. Of the patients, 7 were female and 3 were male. E. coli and Klebsiella pneumonia were most prevalent species. E. coli and Klebsiella pneumonia were multidrug-resistant (resistance to five or more antibiotics) among the isolates. Of the isolated in Uro pathogens such as E. coli and Klebsiella pneumonia were ESBLs positive.Conclusion: Based on the results, E. coli and Klebsiella pneumonia predominated in the samples, giving the maximum frequency of ESBLs producing followed by E. coli (70%) and Klebsiella pneumonia (30%) from the combined disk diffusion method. Herbal medicine should be perform for UTI patients to prevent the spread of more. Only limited therapeutically options for ESBL-PE.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    D, D., Olaganathan, S., & Marimuthu, M. (2021). Detection of Extended Spectrum Beta Lactamases (ESBLs) Producing Enterobacteriaceae Family from Urinary Tract Infection (UTI) Patients. International Journal of Pharmaceutical Investigation, 11(1), 113-117. https://doi.org/10.5530/ijpi.2021.1.21

    Copyright and licence

    © 2021 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0