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Compositional Characterization of Biosurfactant Produced from Pseudomonas aeruginosa ENO14-MH271625 and its Application in Crude Oil Bioremediation

1★, 2, 1, 1★ Corresponding author

  1. 1Department of Microbiology, School of Life Sciences, Pondicherry University, Puducherry, INDIA.
  2. 2Department of Chemistry, School of Physical, Chemical and Applied Sciences, Pondicherry University, Puducherry, INDIA.

CORRESPONDENCE

Ekramul Haque

Department of Microbiology, School of Life Sciences, Pondicherry University, Puducherry, INDIA.

hekramul37@gmail.com

Received: 11-10-2020; Revised: 19-12-2020; Accepted: 01-04-2021.

Volume 11, Issue 2 · pp. 204-207 · 2021 · DOI: 10.5530/ijpi.2021.2.36

ABSTRACT

Objectives: This study aimed to compositionally characterize the biosurfactant and its application in crude oil bioremediation.Methods: Production of biosurfactant was carried out in Bushnell Hass Broth (BHB), supplemented with 1% glucose. Compositional characterizations were performed by 1 H-NMR and ESI-MS analysis. The biodegradation of crude oil was carried out by using a 0.1% (g/v) of purified biosurfactant (ENO14BS). The experiment has three sets namely Set A, Set B and Set C. The experiments are as follows: Set A contained 50 ml sterilized BHB medium with bacterial cells (5% v/v, O.D.600 ~ 1.0), 2% (v/v) of crude oil and ENO14BS (0.1 % w/v). Set B contained 50 ml of sterilized BHB medium with bacterial cells (5% v/v, O.D.600 nm ~ 1.0) and 2% (v/v) of crude oil. Set C (abiotic control) contained 50 ml of sterilized BHB with 2% (v/v) of crude oil only. This experiment was performed for 7 days.Results: Structural elucidation by NMR and Electrospray Ionization-Mass Spectroscopy (ESIMS) showed the presence of six uncommon rhamnolipid homologs, m/z (mass to charge ratio) = 358.98 [M+H]+, Rha-C12:2; m/z = 391.28 [M+H]+, Rha–C6 –C6 ; m/z = 427.38 [M+H]+, Rha–C17:3; m/z = 447.32 [M+H]+, Rha–C8 - C8 ; m/z = 507.22 [M+H]+, Rha-Rha-C12:1; m/z = 648.17 [M+H]+, Rha-RhaC10-C10:1. The application of biosurfactant (ENO14BS) in biodegradation of crude oil was performed at a laboratory scale. The biosurfactant (0.1% w/v) amended microcosm showed up to 73% crude oil degradation (31% higher than with culture alone) in four days.Conclusion: We conclude that ENO14BS biosurfactant produced by Pseudomonas aeruginosa ENO14 using glucose in this study has shown its potential for use in the crude oil bioremediation process.

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    Haque, E., Riyaz, M. A. B., Shankar, S., & Hassan, S. (2021). Compositional Characterization of Biosurfactant Produced from Pseudomonas aeruginosa ENO14-MH271625 and its Application in Crude Oil Bioremediation. International Journal of Pharmaceutical Investigation, 11(2), 204-207. https://doi.org/10.5530/ijpi.2021.2.36

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    © 2021 Author(s)

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