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Detection of Zein Gene of Corn (Zea mays) as Another Material in Arabica Coffee Powder (Coffea arabica) with Gel-based PCR Method

1★, 1, 1★ Corresponding author

  1. 1Faculty of Pharmacy, Bhakti Kencana University, Bandung, West Java, INDONESIA.

CORRESPONDENCE

Soni Muhsinin

Faculty of Pharmacy, Bhakti Kencana University, Bandung, West Java, INDONESIA.

soni.muhsinin@bku.ac.id

Received: 30-12-2022; Revised: 24-12-2023; Accepted: 30-03-2023.

Volume 13, Issue 3 · pp. 512-515 · 2023 · DOI: 10.5530/ijpi.13.3.063

ABSTRACT

Aim/Background: Arabica coffee (Coffea arabica) is the most consumed coffee in the world. As a result, it is susceptible to imitation Arabica coffee made with corn syrup and other inexpensive components (Zea mays). This study detects the corn Zein gene in Arabica coffee powder on the market with the Gel-based PCR method.Materials and Methods: In this study, the samples of research were split into two groups. Samples are separated into two categories: test and control. Controls include unprocessed corn (Zea mays) and coffee beans (Coffea arabica). There are now four distinct varieties of Arabica coffee powder available in the Indonesian market (Coffee arabica). In this study, the simplex PCR method with the primer pair is the Zein gene, and the control primer pair is the ClpP gene as an Arabica coffee gene.Results: The results of this study are that the four test samples show a band parallels to the size of 87 bp (Zein gene band).Conclusion: In conclusion, all test samples (Arabica coffee powder on the market) are suspected of containing the Zein gene, the corn gene.

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    Muhsinin, S., Luthfiyyah, J., & Kusriani, R. H. (2023). Detection of Zein Gene of Corn (Zea mays) as Another Material in Arabica Coffee Powder (Coffea arabica) with Gel-based PCR Method. International Journal of Pharmaceutical Investigation, 13(3), 512-515. https://doi.org/10.5530/ijpi.13.3.063

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    © 2023 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0