Development and evaluation of a novel biodegradable sustained release microsphere formulation of paclitaxel intended to treat breast cancer
1, 1, 2, 3, 1★★ Corresponding author
- 1Departments of Pharmaceutics, Mother Teresa College of Pharmacy, Osmania University.
- 2Pharmacology, Vaagdevi College of Pharmacy Kakatiya University, Hanamkonda, Warangal.
- 3Alliance Institute of Advanced Pharmaceutical and Health Sciences, Jawaharlal Nehru Technical University Hyderabad, Ameerpet, Hyderabad, Andhra Pradesh, INDIA.
CORRESPONDENCE
Jithan Venkata Aukunuru
Departments of Pharmaceutics, Mother Teresa College of Pharmacy, Osmania University.
Volume 3, Issue 3 · pp. 119-125 · 2013 · DOI: 10.4103/2230-973X.119212
ABSTRACT
Objective: The objective of this study was to develop a novel 1 month depot paclitaxel (PTX) microspheres that give a sustained and complete drug release.Materials and Methods: PTX loaded microspheres were prepared by o/w emulsion solvent evaporation technique using the blends of poly(lactic-co-glycolic acid) (PLGA) 75/25, polycaprolactone 14,000 and polycaprolactone 80,000. Fourier transform infrared spectroscopy was used to investigate drug excipient compatibility. Compatible blends were used to prepare F1-F6 microspheres, the process was characterised and the optimum formulation was selected based on the release. Optimised formulation was characterised for solid state of the drug using the differential scanning calorimetry (DSC) studies, surface morphology using the scanning electron microscopy (SEM), in vivo drug release, in vitro in vivo correlation (IVIVC) and anticancer activity. Anticancer activity of release medium was determined using the cell viability assay in Michigan Cancer Foundation (MCF-7) cell line.Results: Blend of PLGA with polycaprolactone (Mwt 14,000) at a ratio of 1:1 (F5) resulted in complete release of the drug in a time frame of 30 days. F5 was considered as the optimised formulation. Incomplete release of the drug resulted from other formulations. The surface of the optimised formulation was smooth and the drug changed its solid state upon fabrication. The formulation also resulted in 1-month drug release in vivo. The released drug from F5 demonstrated anticancer activity for 1-month. Cell viability was reduced drastically with the release medium from F5 formulation. A 100% IVIVC was obtained with F5 formulation suggesting the authenticity of in vitro release, in vivo release and the use of the formulation in breast cancer.Conclusions: From our study, it was concluded that with careful selection of different polymers and their combinations, PTX 1 month depot formulation with 100% drug release and that can be used in breast cancer was developed.
KEYWORDS
REFERENCES
As publishedShowing references and in-text citations exactly as published.
Cite this article
SELECT FORMAT
Shiny, J., Ramchander, T., Goverdhan, P., Habibuddin, M., & Aukunuru, J. V. (2013). Development and evaluation of a novel biodegradable sustained release microsphere formulation of paclitaxel intended to treat breast cancer. International Journal of Pharmaceutical Investigation, 3(3), 119-125. https://doi.org/10.4103/2230-973X.119212
Copyright and licence
© 2013 Author(s)
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0