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Radioprotective effect of hesperidin on reducing oxidative stress in the lens tissue of rats

1, 2, 3, 4, 1, 5, 1

  1. 1Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, IRAN.
  2. 2Department of Medical Physics, Babol University of Medical Sciences, IRAN.
  3. 3Department of Radiology, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, IRAN.
  4. 4Department of Ophthalmology, School of Medicine, Babol University of Medical Sciences, IRAN.
  5. 5Department of Biostatistics and Epidemiology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Mazandaran Province, IRAN.

CORRESPONDENCE

Ali Shabestani Monfared

Department of Medical Physics, Babol University of Medical Sciences, Babol, Mazandaran Province, Iran.

monfared_ali@yahoo.com

Volume 7, Issue 3 · pp. 149-154 · 2017 · DOI: 10.4103/jphi.JPHI_60_17

ABSTRACT

Introduction: Oxidative stress is a common factor in cataract. Considering the antioxidant properties of hesperidin as a flavanone glycoside from the flavonoid family with radioprotective effect, this study aimed to determine the protective effect of this flavanone glycoside on reducing oxidative stress in the eye lens tissue of mature rats caused by gamma irradiation.Materials and Methods: A total of 48 adult rats were randomly divided into six groups, namely, control, Dimethyl sulfoxide (DMSO), hesperidin, radiation, radiation + DMSO, and radiation + hesperidin. 15 Gy irradiation was carried out using Cobalt‑60 teletherapy instrument with a source‑to‑surface distance of 80 cm at a dose rate of 98.5 cGy/min. 2 days following irradiation, we removed the rats’ lenses and analyzed them to determine the effects of hesperidin.Results: The comparison of control and intervention groups after irradiation showed that malondialdehyde (MDA) level in the lens tissue was significantly higher in the irradiation groups than the control group. Furthermore, a significant difference between radiation and radiation + hesperidin groups were observed. The level of glutathione (GSH) in the lens tissue was significantly lower in the irradiation groups compared to the control group. Nonetheless, significant elevation of GSH in the radiation + hesperidin group compared to radiation group was seen.Conclusions: Radiation exposure reduced GSH and enhanced MDA levels in the lens tissue. However, GSH and MDA levels were modulated after hesperidin consumption. These results show the antioxidative properties of hesperidin in the lens and demonstrated that radiation complications such as cataract can be reduced by hesperidin through reducing oxidative stress.

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    Karimi, N., Monfared, A. S., Haddadi, G. H., Soleymani, A., Mohammadi, E., Hajian‑Tilaki, K., & Borzoueisileh, S. (2017). Radioprotective effect of hesperidin on reducing oxidative stress in the lens tissue of rats. International Journal of Pharmaceutical Investigation, 7(3), 149-154. https://doi.org/10.4103/jphi.JPHI_60_17

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    © 2017 Author(s)

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