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Protection of Fargesin against Hydrogen Peroxide-induced Cell Death in RAW 264.7 Cells

1, 1,2, 1,2, 3,5, 3,4, 1,2★★ Corresponding author

  1. 1Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), Bandar Puncak Alam, Selangor, MALAYSIA.
  2. 2Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi MARA Selangor, Puncak Alam, Selangor, MALAYSIA.
  3. 3Center of Excellence in Natural Products for Ageing and Chronic Diseases, Chulalongkorn University, Bangkok, THAILAND.
  4. 4Department of Food and Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, THAILAND.
  5. 5Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, THAILAND.

CORRESPONDENCE

Hasseri Halim

Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), Bandar Puncak Alam, Selangor, MALAYSIA.

hasseri2945@uitm.edu.my

Received: 28-07-2023; Revised: 25-09-2023; Accepted: 25-10-2023.

Volume 14, Issue 1 · pp. 55-61 · 2023 · DOI: 10.5530/ijpi.14.1.8

ABSTRACT

Background: The imbalance level of Reactive Oxygen Species (ROS) in the body can lead to oxidative stress in cells, resulting in the progression of chronic diseases. Fargesin, a bioactive lignan found in Flos magnoliae, an herb widely used in Chinese herbal medicines, has been identified as a potential compound to mitigate this imbalance.Materials and Methods: Hydrogen peroxide (H2 O2), one of the major ROS, is commonly employed as an experimental model to induce oxidative stress. This study aimed to investigate the protective effect of fargesin against cell death induced by hydrogen peroxide in RAW 264.7 macrophage cells. RAW 264.7 macrophage cells were pre-treated with various concentrations of fargesin before being exposed to toxic dose (1 mM or 2 mM) of hydrogen peroxide. The viability of the cells was assessed using the MTT assay, and the morphological changes were described. Results and Discussion: The MTT assay demonstrated that non-toxic doses of fargesin (1.56 µM, 3.13 µM, 6.25 µM, 12.5 µM, and 25 µM) significantly reduced H2 O2 -induced cytotoxicity in the treated cells compared to the control group.Conclusion: This research is expected to provide a basis for future studies in the development of a new antioxidant agent for the treatment and prevention of diseases associated with oxidative stress.

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    Roof, A. N. M., James, R. J., Ramli, S., Sritularak, B., Rojsitthisak, P., & Halim, H. (2023). Protection of Fargesin against Hydrogen Peroxide-induced Cell Death in RAW 264.7 Cells. International Journal of Pharmaceutical Investigation, 14(1), 55-61. https://doi.org/10.5530/ijpi.14.1.8

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    © 2023 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0