Optimization of Lipase Production from Pseudomonas fluorescens
1, 1, 1, 1, 1, 2, 1★★ Corresponding author
- 1Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh, INDIA.
- 2Department of Biotechnology, VFSTR, Guntur, Andhra Pradesh, INDIA.
CORRESPONDENCE
Chandrasekhar Chanda
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh, INDIA.
Received: 21-09-2023; Revised: 11-10-2023; Accepted: 04-11-2023.
Volume 14, Issue 1 · pp. 258-262 · 2023 · DOI: 10.5530/ijpi.14.1.32
ABSTRACT
Objectives: Lipases are widely used in biodiesel, food, detergent, and pharmaceutical industries. To fulfil the rising demand for these crucial industrial enzymes, an extensive study on lipases from various micro-organisms will be useful in maximising the growing conditions and reducing production costs.Materials and Methods: The current work concentrated on optimising the carbon source, inducer concentration, and growth parameters to produce lipases at a reasonable price from pseudomonas species.Results: Extracellular lipases from Pseudomonas fluorescens were produced and optimised, achieving a concentration of 220 IU/mL of submerged fermentation broth.Conclusion: The results showed that the extracellular lipase production from Pseudomonas fluorescens was enhanced by three folds using submerged fermentation.
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Bandi, L., Lakshmisetty, N. S., Ankireddy, K., Pulipati, L. L. V., Bontu, A. L., Aluru, R. R., & Chanda, C. (2023). Optimization of Lipase Production from Pseudomonas fluorescens. International Journal of Pharmaceutical Investigation, 14(1), 258-262. https://doi.org/10.5530/ijpi.14.1.32
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© 2023 Author(s)
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